Complex formation and intermolecular electron transfer between flavocytochrome b2 in the crystal and cytochrome c.
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چکیده
منابع مشابه
Complex formation and intermolecular electron transfer between flavocytochrome b2 in the crystal and cytochrome c.
The present study addresses the question whether tetrameric flavocytochrome b2 in the crystal is catalytically competent and, if so, whether it is possible to prepare a functional complex of the crystalline enzyme with its physiological electron acceptor cytochrome c. By single crystal microspectrophotometry we show that the native reduced enzyme can be oxidized by oxygen or ferricyanide and th...
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15 صفحه اولThe importance of the interdomain hinge in intramolecular electron transfer in flavocytochrome b2.
The two distinct domains of flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase) are connected by a typical hinge peptide. The amino acid sequence of this interdomain hinge is dramatically different in flavocytochromes b2 from Saccharomyces cerevisiae and Hansenula anomala. This difference in the hinge is believed to contribute to the difference in kinetic properties between the two enzym...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)81907-3